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Subcellular fractionation of brown adipose tissue

✍ Scribed by Giacobino, J.-P.

Publisher
Wiley (John Wiley & Sons)
Year
1979
Tongue
English
Weight
320 KB
Volume
11
Category
Article
ISSN
0091-7419

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✦ Synopsis

The present study proposes a technique, using Metrizamide, which permits the preparation of brown adipose tissue plasma membranes from the crude mitochondria as well as from the crude microsome fraction. These plasma membranes have high relative specific activities of their marker enzyme, 5'-nucleotidase (1 5 f 3 and 14 f 2 respectively) and, particularly those originating in the crude microsomes, are relatively free of mitochondria contamination. This study also shows the influence of the mode of cell disruption on microsome integrity. When cell disruption was achieved by grinding in liquid nitrogen the purified microsome NADPH cytochrome c reductase specific activity was found to be 3.5 times greater than that of microsomes obtained after homogenization of the tissue. Key words: subcellular fractionation,

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This study was supported by the Deutsche For-mediately after excision in ice-cold 0.25 N schunasgemeinschaft (Ra 231/4-S). NaOH to a concentration of 15-25 mg/ml ' F& definition of structural and functional differences of adi pose tissues see (IO). ' Abbreviation used: PCA, perchloric acid.