✦ LIBER ✦

Subattomole detection of amino acids by capillary electrophoresis based on semiconductor laser fluorescence detection

✍ Scribed by Tetsuhiro Fuchigami; Totaro Imasaka; Masanobu Shiga

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 351 KB
Volume: 282
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(93)80370-z

No coin nor oath required. For personal study only.

✦ Synopsis

A pyronin succinimidyl ester fluorescent in the deep-red region is synthesized, whose absorption maximum and molar absorptivity are 663 nm and 8.2 X 104, respectively. This compound is used for fluorescence labeling of amino acids such as alanine, arginine, glycine, glutamic acid, and aspartic acid. The sample containing the labekd amino acids is separated by capillary electrophoresis and is detected by semiconductor laser Buorometry. A sheath flow cell is used for reduction of the light scatter from the cell wall. This compact and inexpensive analytical system allows detection of 0.8-4.5 attomole of amino acids.

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