## Abstract The inhibition of D‐amino acid oxidase contained in permeabilized cells of the yeast __Trigonopsis variabilis__ by α‐keto acids (pyruvic acid, phenylpyruvic acid and 4‐methylthio‐2‐oxobutanoic acid), products of the transformation of the corresponding D‐amino acids, was studied. In all
Study on the synthesis of D-amino acid oxidase in Trigonopsis variabilis in continuous culture
✍ Scribed by Chia-Jenn Wei; Jen-Chau Huang; Yi-Po Tsai
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 418 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0006-3592
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📜 SIMILAR VOLUMES
## Background Immobilization of __Trigonopsis variabilis__ D-amino acid oxidase (__Tv__DAO) on solid support is the key to a reasonably stable performance of this enzyme in the industrial process for the conversion of cephalosporin C as well as in other biocatalytic applications. ## Results To pr
## Abstract Covalent modification of purified __Trigonopsis variabilis__ D‐amino acid oxidase using maleimide‐activated poly(ethylene glycol) 5000 yielded a stable bioconjugate in which three surface‐exposed cysteine side chains were selectively derivatized. Compared with the native enzyme, the PEG