𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Study on proteins from yeast cytoplasmic ribosomes by two-dimensional gel electrophoresis

✍ Scribed by Ishiguro, Junpei

Publisher
Springer
Year
1976
Tongue
English
Weight
441 KB
Volume
145
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

Proteins of yeast cytoplasmic ribosomes were analyzed by two different methods of two dimensional gel electrophoresis: run at pH 8.6 in 1-D1 and at pH 4.6 in 2-D (Method A); run at pH 5.0 in 1-D and in the presence of sodium dodecyl sulfate in 2-D (Method B). The numbers of proteins estimated were 28 (Method A) and 29 or 30 (Method B) in the 40S small subunit, and 40 (Method A) and 41 (Method B) in the 60S large subunit, respectively. Molecular weights of proteins in the small and the large subunits were found to be less than 40,000 and 60,000 respectively.

πŸ“œ SIMILAR VOLUMES

Characterisation of the ribosomal protei
Characterisation of the ribosomal proteins from Schizosaccharomyces pombe by two-dimensional polyacrylamide gel electrophoresis
✍ Coddington, Alan ;Fluri, Rudolf πŸ“‚ Article πŸ“… 1977 πŸ› Springer 🌐 English βš– 740 KB

The 80S ribosome of Schizosaccharomyces pombe contains 93 proteins as determined by twodimensional electrophoresis on polyacrylamide gels. Of these, 76 are basic and 17 are acidic at pH 8.7. 38 proteins could be assigned unambiguously to the large sub-unit and 19 to the small. 67 proteins were extra

Analysis of mitochondrial ribosomal prot
Analysis of mitochondrial ribosomal proteins of Saccharomyces cerevisiae by two dimensional polyacrylamide gel electrophoresis
✍ Faye, GοΏ½rard ;Sor, FrοΏ½dοΏ½ric πŸ“‚ Article πŸ“… 1977 πŸ› Springer 🌐 English βš– 664 KB

Proteins from mitochondrial ribosomes of Saccharomyces cerevisiae were analysed by a two dimensional gel electrophoresis method. Each ribosomal subunit revealed a reproducible characteristic pattern of protein components. The 37S small subunit contained 33 protein species with an average molecular w