Study of the Ribonuclease−S-Protein−Peptide Complex Using a Radical Probe and Electrospray Ionization Mass Spectrometry
✍ Scribed by Wong, Jason W. H.; Maleknia, Simin D.; Downard, Kevin M.
- Book ID
- 120349736
- Publisher
- American Chemical Society
- Year
- 2003
- Tongue
- English
- Weight
- 315 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0003-2700
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The thermal stability of ribonuclease S (RNase S), an enzymatically active noncovalent complex composed of a 2166-u peptide (S-peptide) and a 11,534-u protein (S-protein), was investigated by electrospray ionization mass spectrometry (ESI-MS) and capillary electrophoresis ESI-MS (CE-ESI-MS). The int