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Electrospray Ionization Mass Spectrometry: A Promising New Technique in the Study of Protein/DNA Noncovalent Complexes

✍ Scribed by Timothy D. Veenstra


Book ID
115581558
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
67 KB
Volume
257
Category
Article
ISSN
0006-291X

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## Abstract Electrospray ionization time‐of‐flight mass spectrometry (ESI‐TOF MS) has been used to study noncovalent interactions between the __trp__ apo‐repressor (TrpR), its co‐repressor tryptophan and its specific operator DNA. In 5 mM ammonium acetate, TrpR was detected as a partially unfolded

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The detection of non-covalent complexes in the mass range 19 000-34 000 Da, using electrospray ionization mass spectrometry (ESI-MS), is reviewed. The examples discussed include (1) a protein-ligand interaction (ras-GDP), (2) an inhibitor-protein-ligand interaction (SCH 54292/SCH 54341-ras-GDP), (3)