Electrospray Ionization Mass Spectrometry: A Promising New Technique in the Study of Protein/DNA Noncovalent Complexes
β Scribed by Timothy D. Veenstra
- Book ID
- 115581558
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 67 KB
- Volume
- 257
- Category
- Article
- ISSN
- 0006-291X
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## Abstract Electrospray ionization timeβofβflight mass spectrometry (ESIβTOF MS) has been used to study noncovalent interactions between the __trp__ apoβrepressor (TrpR), its coβrepressor tryptophan and its specific operator DNA. In 5 mM ammonium acetate, TrpR was detected as a partially unfolded
The detection of non-covalent complexes in the mass range 19 000-34 000 Da, using electrospray ionization mass spectrometry (ESI-MS), is reviewed. The examples discussed include (1) a protein-ligand interaction (ras-GDP), (2) an inhibitor-protein-ligand interaction (SCH 54292/SCH 54341-ras-GDP), (3)