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Study of the ‘Raman spectroscopic signature of life’ in mitochondria isolated from budding yeast

✍ Scribed by Liang-da Chiu; Masahiro Ando; Hiro-o Hamaguchi

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
117 KB
Volume
41
Category
Article
ISSN
0377-0486

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✦ Synopsis

Abstract

The ‘Raman spectroscopic signature of life’ is a Raman band at 1602 cm^−1^ that sharply reflects the metabolic activity of cell mitochondria. Here we report the study of thissignature in isolated yeast mitochondria. The existence and behaviour of the 1602 cm^−1^ band in isolated mitochondria have been confirmed to be the same as in living yeast cells: the intensity of the band decreases with timewhen a respiration inhibitor, sodium azide, is added. The present study shows the significance of isolated mitochondria in elucidating the origin of this still unassigned Raman band. Copyright © 2009 John Wiley & Sons, Ltd.

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