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Study of the interaction between ofloxacin and human serum albumin by spectroscopic methods

✍ Scribed by Aurica Varlan; Sorana Ionescu; Mihaela Hillebrand

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
396 KB
Volume
26
Category
Article
ISSN
1522-7235

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✦ Synopsis

ABSTRACT

The binding of ofloxacin (OFLX) to human serum albumin (HSA) was investigated by fluorescence and circular dichroism (CD) techniques. The binding parameters have been evaluated by a fluorescence quenching method. Competitive binding measurements were performed in the presence of warfarin and ibuprofen and suggest binding to the warfarin site I of HSA. The distance r between donor (HSA) and acceptor (OFLX) was estimated according to the Forster's theory of non‐radiatiative energy transfer. CD spectra revealed that the binding of OFLX to HSA induced conformational changes in HSA. Molecular docking was performed and shows that for the lowest energy complex OFLX is located in site I of HSA, which correlate to the competitive binding experiments. Copyright © 2011 John Wiley & Sons, Ltd.

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