𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Study of the effect of organic solvents on the synthesis of levan and the hydrolysis of sucrose by Bacillus subtilis levansucrase

✍ Scribed by Régis Chambert; Marie-Françoise Petit-Glatron

Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
559 KB
Volume
191
Category
Article
ISSN
0008-6215

No coin nor oath required. For personal study only.

✦ Synopsis

The equilibrium between the hydrolase and synthetase activities of levansucrase was determined by progressively substituting water with various organic solvents in the enzymic reaction medium. In the presence of high concentrations of these solvents, the enzyme displayed only synthetase activity. The levan obtained under such conditions had M, -106 and presented a low molecular dispersity. In the presence of solvent, the K, values for sucrose and raffinose remained unchanged, but the k,,, values were five times higher in compa~son to the same constants determined for an aqueous medium.

📜 SIMILAR VOLUMES

The influence of dissolved oxygen tensio
The influence of dissolved oxygen tension on the synthesis of the antibiotic difficidin by bacillus subtilis
✍ M. Suphantharika; A. P. Ison; M. D. Lilly; B. C. Buckland 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 491 KB 👁 2 views

## Abstract The antibiotic, difficidin, and its hydroxylated derivative oxydifficidin, were synthesized by cultures of __Bacillus subtilis__ grown on a complex medium. Maximum titers of about 200 and 130 mg/L, respectively, were obtained. In fermentations where the dissolved oxygen tension (DOT) wa

The effect of sucrose hydrolysis on the
The effect of sucrose hydrolysis on the stability of protein therapeutics during accelerated formulation studies
✍ Douglas D. Banks; David M. Hambly; Joanna L. Scavezze; Christine C. Siska; Nicol 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 185 KB 👁 1 views

Stability studies of protein therapeutics are often accelerated by storing potential formulations at elevated temperatures where the rates of various chemical and physical degradation pathways are increased. An often overlooked caveat of using these studies is the potential degradation of the formul