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Study of the crystal shape and its influence on the anti-tumor activity of tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL)

✍ Scribed by Ji-Cheng Yin; Jin-Song Zhou; Jing Sun; Yi Qiu; Dong-Zhi Wei; Ya-Ling Shen

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
320 KB
Volume
43
Category
Article
ISSN
0232-1300

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✦ Synopsis

Abstract

This paper describes a method about the crystal shape control improving the anti‐tumor activity of tumor necrosis factor‐related apoptosis‐inducing ligand (Apo2L/TRAIL) in a batch cooling suspension crystallization by selecting pH values as a controllable variable. Three shaped TRAIL crystals could be obtained under different pH conditions, among which the hexagonal plate crystals had the highest specific activities against tumor cell line. The relationship among pH values, trimer contents and the specific activities of crystals and the purified TRAIL solutions were investigated. The results showed that different trimer contents resulted from pH altering in crystals and protein solutions is a main reason for their different specific activities. The studies may supply a new method to improve the bioactivity of TRAIL agent during its production and storage. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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