Study of ribosomal deoxyribonucleic acid of sea urchin
β Scribed by Nirmal Kumar Mishra
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 333 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0301-4851
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The ribosomal RNA genes from the sea urchin Lytechinus variegatus have been studied with the electron microsocope using the technique of denaturation mapping. A repeating pattern of denatured regions was found with an average repeat length of 3.87 +/- 0.24 mum. This corresponds to a DNA sequence of
Lysis of mitochondria from sea urchin embryos with Triton X-100 led to a complete conversion of DNA-containing mitochondrial residues into protein-DNA complex with a density higher than 1.22 g/cm3 in sucrose solutions. This complex banded isopycnically in metrizamide gradients at a density of 1.26 g
The base composition of D S A from microorganisms varies over a wide range of guanine + cytosine (G-C) content.. We have examined the infrared spectra of a number of DNA samples isolated from different microorganisms and have found marked differences in their infrared spectra. These differences have