Infrared studies of deoxyribonucleic acids, their constituents and analogues. II. Deoxyribonucleic acids with different base composition
✍ Scribed by H. Fritzsche
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1967
- Tongue
- English
- Weight
- 450 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
The base composition of D S A from microorganisms varies over a wide range of guanine + cytosine (G-C) content.. We have examined the infrared spectra of a number of DNA samples isolated from different microorganisms and have found marked differences in their infrared spectra. These differences have been related to the G-C content of the investigated DNA samples. A comparison between the infrared spectra of nucleosides, synthetic polynucleotides, apurinic acid, and apyrimidinic acid has permitted a more extensive assignment of infrared bands to distinct base residues. The relative intensity of the absorption bands a t 1485 and 1505 cm.-l depends on the G-C content of the deuterated DNA sample. On the basis of these results a new method for the determination of the G-C content in DNA has been developed from the results. Its advantage is that the measurement is rapid and the DNA samples are not changed by the procedure.