Study of intracellular localization of the proteolytic enzyme complex and its protein inhibitors in bombyx grain
✍ Scribed by D. V. Yarygin, N. O. Min’kova…
- Book ID
- 120770803
- Publisher
- Springer
- Year
- 2012
- Tongue
- English
- Weight
- 140 KB
- Volume
- 43
- Category
- Article
- ISSN
- 1062-3604
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## Abstract The potential of electrospray ionization (ESI) mass spectrometry (MS) to detect non‐covalent protein complexes has been demonstrated repeteadly. However, questions about correlation of the solution and gas‐phase structures of these complexes still produce vigorous scientific discussion.
Rhizopus nigricans ATCC 6227b grown in either shake flasks or a fermentor was sheared in a concentric cylinder viscometer. The cells grown in shake flasks were found to be more susceptible to disruption by shear than those grown in the fermentor. Cells resuspended in a medium containing reduced glut