Effect of shear on membrane-associated enzymes: Studies of the release of intracellular protein and of the progesterone 11α-hydroxylase complex from rhizopus nigricans

✍ Scribed by B. L. Talboys; P. Dunnill

• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 417 KB
• Volume: 27
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260271216

✦ Synopsis

Rhizopus nigricans ATCC 6227b grown in either shake flasks or a fermentor was sheared in a concentric cylinder viscometer. The cells grown in shake flasks were found to be more susceptible to disruption by shear than those grown in the fermentor. Cells resuspended in a medium containing reduced glutathione and EDTA were found to be more easily disrupted than cells resuspended in 0.5°/0 NaCI. The optimum condition for disruption of shake flask cells grown in the former medium with retention of progesterone 1 la-hydroxylase activity was a laminar shear rate of 4300 s-', for a period of 3 min at 4°C. During the first 30 s the apparent viscosity was found to decrease significantly with applied shear.