Effect of shear on membrane-associated enzymes: Studies of isolated progesterone 11α-hydroxylase complex from Rhizopus nigricans

✍ Scribed by B. L. Talboys; P. Dunnill

• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 326 KB
• Volume: 27
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260271215

✦ Synopsis

Previously published work concerned with the effect of laminar shear on enzymes has been centred on water-soluble proteins.I4 Although there are divergent views about their stability, some studies have shown water soluble enzymes to be robust under the application of very high shear field^.^,^ In a preceding arti~le,~ we reported on the release of the progesterone l l a hydroxylase complex from Rhizopus nigricans by applied shear. The results suggested that the enzyme complex was much more susceptible to applied shear than soluble globular proteins. However, the occurrence together of cell disruption by shear forces and shearassociated changes in the activity of the enzymemembrane complex did not permit a detailed analysis of the latter effect. Therefore, this communication describes shear experiments on the complex freed from cell debris.