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Study of histone—histone interactions by affinity chromatography

✍ Scribed by J. Mizon; M. Mayne-D'Haultfoeuille; C. Mizon-Capron; P. Sautiere; G. Biserte


Book ID
115904466
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
240 KB
Volume
47
Category
Article
ISSN
0014-5793

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A study of histone-histone interactions
✍ A. L. Jasinskas; A. A. Gineitis 📂 Article 📅 1976 🏛 Springer 🌐 English ⚖ 372 KB

Homologous whole histone from calf thymus was adsorbed on Sepharose 4B columns with covalently coupled histone fractions H2a, H2b, H3 or H4 in 0.01 M phosphate buffer, pH 6.7 - 1 M NaCl. The adsorbed histones were eluted from the columns with 5 M urea in the same buffer. Electrophoretic analysis has