Study of cell kinetics by computer-analyzed flow cytometric histograms

✍ Scribed by A. Bertuzzi; A. Gandolfi; A. Germani; R. Vitelli; G. Badaracco; G. Starace

• Book ID: 103930355
• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 481 KB
• Volume: 14
• Category: Article
• ISSN: 0898-1221
• DOI: 10.1016/0898-1221(87)90225-2

✦ Synopsis

A mathematical procedure for analyzing the cell proliferation kinetics from DNA content histograms, measured by flow cytometry, is presented. This procedure is based on a cell cycle model which uses a continuity equation for the S-phase transit. Four tumor cell lines in culture were studied. A human melanoma line (JR01) was examined in exponential growth. For another human melanoma (MI4) and a human astrocytoma (DF) lines, a sequence of flow cytometric histograms with the corresponding growth curve was processed, determining the rate of DNA synthesis and the S-phase cell influx. The same kinetic parameters were evaluated also in a murine tumor line (3LL C!08) treated with an antineoplastic drug (ICRF 159).