Study of amino and sulfhydryl sites in the sodium pathway in dog red blood cell membranes
β Scribed by V. Castranova; P. R. Miles
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 929 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0022-2631
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We have compared properties of the red blood cell Ca2(+)-ATPase in two types of preparations: red cell membrane ghosts (enzyme in unfractionated membranes) and after purification (detergent-soluble enzyme). The Ca2(+)-ATPase activity was studied with respect to its requirement for: calmodulin, calci
## Abstract It is shown that the signal of intracellular and extracellular sodium of red blood cells can be separated by a difference in diffusion. Comparison with proton diffusion experiments conducted in parallel showed that this difference was caused by restriction to the cell volume. The measur
Carnitine is bound by intact red blood cells, by red blood cell ghosts, and by glutaraldehyde-fixed human erythrocytes in a non-saturable, temperaturedependent manner. Binding of carnitine by these preparations is blocked by sulfhydryl reagents. Incubation or preincubation of red blood cell preparat