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Studies on two major contaminating proteins of the cytoplasmic inclusion bodies in Escherichia coli

✍ Scribed by Kannappan Veeraragavan


Book ID
109314973
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
271 KB
Volume
61
Category
Article
ISSN
0378-1097

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Chemical treatment of Escherichia coli.
✍ Robert J. Falconer; Brian K. O'Neill; Anton P. J. Middelberg πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 161 KB πŸ‘ 2 views

A method is presented for the direct extraction of the recombinant protein Long-R 3 -IGF-I from inclusion bodies located in the cytoplasm of intact Escherichia coli cells. Chemical treatment with 6M urea, 3 mM EDTA, and 20 mM dithiothreitol (DTT) at pH 9.0 proved an effective combination for extract

Chemical treatment of Escherichia coli:
✍ Robert J. Falconer; Brian K. O'Neill; Anton P. J. Middelberg πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 308 KB πŸ‘ 2 views

In previous parts of this study we developed procedures for the high-efficiency chemical extraction of soluble and insoluble protein from intact Escherichia coli cells. Although high yields were obtained, extraction of recombinant protein directly from cytoplasmic inclusion bodies led to low product