A method is presented for the direct extraction of the recombinant protein Long-R 3 -IGF-I from inclusion bodies located in the cytoplasm of intact Escherichia coli cells. Chemical treatment with 6M urea, 3 mM EDTA, and 20 mM dithiothreitol (DTT) at pH 9.0 proved an effective combination for extract
β¦ LIBER β¦
Studies on two major contaminating proteins of the cytoplasmic inclusion bodies in Escherichia coli
β Scribed by Kannappan Veeraragavan
- Book ID
- 109314973
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 271 KB
- Volume
- 61
- Category
- Article
- ISSN
- 0378-1097
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