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Studies on the synthesis of 5-amino-4-imidazolecarboxamide ribotide by cell-free extracts of Escherichia coli

โœ Scribed by Samuel H. Love; Paul D. Boyles


Book ID
115788377
Publisher
Elsevier Science
Year
1959
Weight
430 KB
Volume
35
Category
Article
ISSN
0006-3002

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Synthesis of 14C-uridine-5โ€ฒ -triphosphat
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## Abstract Crude cellโ€free extracts of Escherichia coli AB 259 Hfr 3000, thiโ€perform multistep conversion of ^14^Cโ€uracil to ^14^Cโ€UTP in vitro in the presence of Dโ€ribose/or riboseโ€5โ€phosphate/, ATP and ATPโ€regenerating system. ATP concentration is crucial for significant ^14^Cโ€UTP production. Qu