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Studies on the specific activity of [γ-32P]ATP in adipose and other tissue preparations incubated with medium containing [32P]phosphate

✍ Scribed by Timothy J. Hopkirk; Richard M. Denton


Book ID
119120269
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
813 KB
Volume
885
Category
Article
ISSN
0167-4889

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A new, rapid method for the measurement of [y3\*P]ATP specific radioactivity in tissue extracts in the presence of other 32P-containing compounds is described. The deproteinized extract is incubated with phosphorylase b and phosphorylase kinase, and the incorporation of 3zP into protein from [Y-~\*P