The O-antigen from Vibrio choleme 0:21 has been investigated, using n.m.r. spectroscopy, methylation analysis, and Smith degradation as the main methods. It is concluded that the O-antigen is composed of tetrasaccharide repeating-units having the following structure (in which Hep = D-glycero-D-manno
Studies on the partial structure of the O-antigen of Vibrio cholera ogawa G-2102
β Scribed by Mita Majumder; Amal K. Mukherjee; Bhakti Guha Thakurta; Archana Dutta; Dipti Sasmal
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 536 KB
- Volume
- 122
- Category
- Article
- ISSN
- 0008-6215
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β¦ Synopsis
Detailed information was obtained regarding the partial structure of the lipopolysaccharide (LPS), containing glucose, glucuronic acid, 2-amino-2-deoxy-glucose, L-glycero-D-gluco-heptose, and small proportions of L-glycero-D-manno-heptose, mannose, and galactose, isolated from Vibrio cholera Ogawa G-2102. Structures of three oligosaccharides were determined. Results of deamination experiments established the sequence of the linkages between the amino sugar and heptose residues in the O-antigenic polysaccharide.
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The 0-polysaccharide from k%rio cholerae 0 : 5 has been investigated, using NMR spectroscopy as the main method. Fast atom bombardment mass spectrometry (FABMS) studies of fragments obtained on treatment with anhydrous hydrogen fluoride or methanolic hydrogen chloride gave further structural informa
The structure of the Vibrio cholerae O:3 O-antigen polysaccharide has been investigated, mainly by n.m.r. spectroscopy, mass spectrometry, sugar and methylation analysis, and specific degradations, and is proposed to involve the following tetrasaccharide repeating-unit. [formula: see text]. In this
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