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Studies on the mechanisms of invasion in cancer. III. Purification of a neutral protease of rat ascites hepatoma cell associated with production of chemotactic factor for cancer cells

✍ Scribed by Masashi Koono; Kenichiro Ushijima; Hideo Hayashi

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
806 KB
Volume
13
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

A neutral protease was extracted from rat ascites hepatoma cells and effectively purified by fractionation with ammonium sulfate followed by chromatography using TEAE‐cellulose and hydroxylapatite in that order. The protease eluted in 120 mM phosphate buffer (pH 6.8) on hydroxylapatite, on experimental injection, produced locally a factor chemotactic for cancer cells and induced an extravascular emigration of circulating tumor cells followed by a formation of metastatic secondary tumors. It was SH‐independent or metal‐independent. The separation of the protease from nucleic acid was performed by disc electrophoresis. By contrast, another neutral protease, alkaline protease and acid protease from the same cells were negative in causing an extravascular emigration of circulating tumor cells.

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