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Studies on the mechanisms of invasion in cancer. I. Isolation and purification of a factor chemotactic for cancer cells

✍ Scribed by Kenshi Yoshida; Teruhisa Ozaki; Kenichiro Ushijima; Hideo Hayashi

Publisher
John Wiley and Sons
Year
1970
Tongue
French
Weight
695 KB
Volume
6
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

A substance chemotactic for cancer cells was extracted from the pseudoglobulin fraction of some tumor tissues of animal and human origin. It was highly purified by column chromatography using Sephadex G‐50 and CM‐Sephadex and then by disc electrophoresis; it behaved as a homogenous substance on disc electrophoresis. The substance was a protein with a molecular weight of approximately 70,000. It was thermolabile and had no proteolytic activity. The material was similarly active for rat ascites hepatoma AH109A cells, mouse ascites hepatoma MH134 cells and mouse myeloid leukemia C‐1498 cells, suggesting a common chemotactic action. It was ineffective for polymorphonuclear leukocytes of rats. No such chemotactic activity was demonstrated by the protein fraction from normal skin and muscle.

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