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Studies on the incorporation of isotopically labeled nucleotides and amino acids in planaria

✍ Scribed by Best, Jay Boyd ;Rosenvold, Robert ;Souders, Jacqueline ;Wade, Carlton

Publisher
John Wiley and Sons
Year
1965
Tongue
English
Weight
617 KB
Volume
159
Category
Article
ISSN
0022-104X

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✦ Synopsis

Neither intact nor regenerating planaria incorporated any significant amount of tritiated thymidine, tritiated L-leucine, or Ci4-L-leucine from free solution even over long periods of exposure. Tritiated thymidine was incorporated into regenerating rat liver and fed to planaria, but tritium hydrogen exchange rendered this method useless. A large incorporation of Ci4-uracil and Ci4-L-leucine into planaria was obtained by growing baker's yeast in a defined medium containing the labeled compound and then feeding the acetone dried yeast to the planaria. Ci4 assay of the amino acid components of planaria fed on Ci4-L-leucine labeled yeast showed Ci4 in only the L-leucine component and yielded a minimum estimate for protein turnover of 46% in seven weeks. Radioautographs of planaria fed on Ci4-uracil labeled yeast exhibited specific incorDoration into ribonucleic acid. Previous radioisotopic studies on planaria are evaluated and criticized. protein was determined.

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