Studies on the heterogeneity of human serum Lp lipoproteins and on the occurrence of double Lp lipoprotein variants

✍ Scribed by Nancy Reid Harvie; Jane S. Schultz

• Publisher: Springer
• Year: 1973
• Tongue: English
• Weight: 600 KB
• Volume: 9
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00485737

✦ Synopsis

Lp lipoproteins have been prepared by a mild method from the serum of a large number of individuals. Approximately 25%o of the individuals tested showed the presence of a double Lp peak in analytical ultracentrifuge diagrams. These double peaks were designated Lp(a)-I and Lp(a)-2 to distinguish them from the single Lp(a) peak. The mean viscosity-corrected sedimentation coefficient, sl.oo4, 20 c, and density of the single Lp(a) peak were 15.8 +_ 1.8s (n = 32) and 1.076++_0.01 g/ml, of the Lp(a)-I peak were 13.5+_1.1s (n = 14) and 1.064+ 0.007 g/ml, and of the Lp(a)-2 peak were 16.8 +-1.7s (n = 14) and 1.074 +-0.009 g/ml. Absorption tests using a double and single Lp preparation showed that both Lp peaks in the double variants possess Lp(a) specificity. Evidence is lacking as yet for individual specificities for either Lp(a)-I or Lp(a)-2. Interand intra-individual heterogeneity among Lp lipoproteins is discussed.