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Studies on the cell-wall mannan of the siphonous green algae, Codium latum

✍ Scribed by Shinobu Kaihou; Tatsuyuki Hayashi; Osamu Otsuru; Masaakira Maeda

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 882 KB
Volume: 240
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(93)84184-8

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✦ Synopsis

Cell-wall mannan from Codium latum was completely solubilized as methylol mannan from their microfibrils by treatment with paraformaldehyde-Me,SO systems at 150 "C, and it was purified by gel-permeation chromatography with Toyopearl gel under Me,SO elution. Mannan was regenerated by dilution of the purified methylol mannan with water or methanol. The elution profile of regenerated mannan on gel permeation chromatography under Me,SO or the IR spectrum of the deuterium-exchanged, fractionated mannan revealed that the cell-wall mannan was aggregated as higher molecular weight material through intermolecular hydrogen bonding to form a difficultly soluble material. The results of structural determination on the purified mannan, including periodate oxidation and permethylation analysis, revealed a (1 + 4)-p-linked linear polysaccharide, as well as other cell-wall microfibril polysaccharides.

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