Chemical characteristics of insoluble glucans from the cell wall of the marine green alga Ulva lactuca (L.) Thuret

✍ Scribed by M. Lahaye; D. Jegou; A. Buleon

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 806 KB
• Volume: 262
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(94)84008-3

✦ Synopsis

The chemical composition and structure of the glucan in the alkali-insoluble cell-wall polysaccharides from the marine green seaweed Ulva lactucu are reported. The cr-cellulose is composed (in mol%) of glucose (72.91, xylose (9.71, rhamnose (4.61, uranic acid (4.3), and sulfate (8.5). The last three components do not appear to be part of the glucan as they were partially removed by methylmorpholine N-oxide treatment. Enzymatic hydrolysis with two commercial glucanases containing preparations generated short oligosaccharides and sugars that were identified by chemical analyses and NMR spectroscopy as glucose, xylose, glucitol, cellobiose, and a new trisaccharide identified as /?-o-xylopyranosyl-(1 + 4)-f&~glucopyranosyl-0 + 4)-o-glucopyranose. The amorphous-like cellulose X-ray diffraction diagram obtained from the alkali-insoluble and enzyme resistant polymers was interpreted in the light of the presence of xylose-containing glucan in the insoluble cell-wall polysaccharides from U. lactucu.