Cell-wall polysaccharides from the marine green alga Ulva “rigida” (ulvales, chlorophyta). Extraction and chemical composition
✍ Scribed by B. Ray; M. Lahaye
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 610 KB
- Volume
- 274
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
Cell-wall polysaccharides from the green seaweed Ulva "rigida" were extracted sequentially with oxalate, 1 and 4 M KOH, sodium chlorite and 4 M KOH again. The chemical composition of the soluble and insoluble extracts and of the DEAE-Sepharose CL-6B fractions of the soluble polysaccharide were determined. Three main types of polysaccharide families were solubilized. The major family was composed of sulfated glucuronorhamnoxyloglycans (ulvan) and was essentially extracted with oxalate. Two hemicellulosic fractions were also isolated and consisted of glucuronans and glucoxylans. Minor fractions consisting of sulfated polysaccharides containing glucose, xylose, mannose and protein were also isolated.
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In order to obtain information on sugar sequences in the water soluble polysaccharides from Ulva "rigida", oligosaccharides were purified by anion exchange and gel permeation chromatography from the partial acid hydrolysate of the native or desulfated ulvan. The chemical structure and sugar sequence
The chemical composition and structure of the glucan in the alkali-insoluble cell-wall polysaccharides from the marine green seaweed Ulva lactucu are reported. The cr-cellulose is composed (in mol%) of glucose (72.91, xylose (9.71, rhamnose (4.61, uranic acid (4.3), and sulfate (8.5). The last three