A rapid method for distinction between gram-negative and grampositive bacteria by means of a 3% solution of potassium hydroxide is tested on 71 gram-positive and 55 gram-negative bacterial strains. The method proved reliable with one exception only, a Bacillus macerans strain. That strain was defina
Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria
β Scribed by G. Cerny
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 495 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1432-0614
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β¦ Synopsis
The aminopeptidase test was performed with representatives of gram-negative, gram-positive, and gram-variable bacteria. All gram-negative bacteria tested gave a positive test reaction with L-alanine-4-nitroanilide as test substrate. Representatives of the coryneform bacteria and some streptococci showed aminopeptidase activity after prolonged reaction times. A correlation between aminopeptidase activity and distinct interpeptide bridge composition in the peptidoglycan of many strains was demonstrated. The influence of growth conditions on aminopeptidase activity of intact bacterial cells is shown.
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