𝔖 Bobbio Scriptorium

✦ LIBER ✦

Studies on the active site of E. coli RNA polymerase

✍ Scribed by GuntherL. Eichhorn; Peter Chuknyisky; Richard Beal; Joseph Rifkind; Raj Pillai; Edward Tarien

Publisher: Elsevier Science
Year: 1989
Tongue: English
Weight: 86 KB
Volume: 36
Category: Article
ISSN: 0162-0134
DOI: 10.1016/0162-0134(89)84573-8

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Genetics studies on the β subunit of Esc

Genetics studies on the β subunit of Escherichia coli RNA polymerase

✍ Nene, Vishvanath ;Glass, Robert E. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 486 KB

A single species of RNA polymerase is responsible for the transcription of genetic material in Escherichia coli; and, each component of this essential enzyme is encoded by a single gene. Nevertheless, we report the characterisation of a viable deletion mutant lacking about 165 bp in the region codin

Genetic studies on the β subunit of Esch

Genetic studies on the β subunit of Escherichia coli RNA polymerase

✍ Nene, Vishvanath ;Glass, Robert E. 📂 Article 📅 1982 🏛 Springer 🌐 English ⚖ 659 KB

Genetic studies on the β subunit of Esch

Genetic studies on the β subunit of Escherichia coli RNA polymerase

✍ Nene, Vishvanath ;Glass, Robert E. 📂 Article 📅 1982 🏛 Springer 🌐 English ⚖ 544 KB

Genetic studies on the β subunit of Esch

Genetic studies on the β subunit of Escherichia coli RNA polymerase

✍ Nene, Vishvanath ;Glass, Robert E. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 713 KB

We have isolated a set of strains that synthesise 331 potential variants of E. coli DNA-dependent RNA polymerase making use of nonsense suppression of amber mutations in the beta structural gene; rpoB. Translational mapping, together with the effect of known amino acid substitutions, has allowed us

Genetic studies on the β subunit of Esch

Genetic studies on the β subunit of Escherichia coli RNA polymerase

✍ Glass, Robert E. ;Jones, Steven T. ;Ishihama, Akira 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 458 KB

RNA polymerase was isolated from two suppressed rpoB amber strains exhibiting relaxed control over RNA synthesis in vivo. In vitro transcription analysis of these mutant holoenzymes carrying defined substitutions at known sites in the beta subunit clearly shows that single amino acid changes in beta

Genetic studies on the β subunit of Esch

Genetic studies on the β subunit of Escherichia coli RNA polymerase

✍ Boothroyd, Clare M. ;Malet, Rosemary M. ;Nene, Vishvanath ;Glass, Robert E. 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 393 KB

Spontaneously-occurring rifampicin-resistant mutants that survive on low (20 micrograms ml-1) but not high drug concentrations (200 micrograms ml-1) have been isolated. One such mutation appears to map close to residue 650 on the beta structural gene. RNA polymerase from low-level resistant strains