Studies on temperature-sensitive mutants of Chinese hamster ovary cells affected in DNA synthesis
✍ Scribed by P. R. Srinivasan; Radhey S. Gupta; Louis Siminovitch
- Book ID
- 112558717
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 708 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1572-9931
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📜 SIMILAR VOLUMES
## Abstract A temperature‐sensitive (ts) mutant of Chinese hamster cells (K12) is blocked at a step late in G~1~, required for the initiation of DNA synthesis. The ts lesion is recessive in interspecific hybrids.
## Abstract We have analyzed ongoing DNA replication in __ts__ BN‐2, a __dna__^−^ mutant of BHK‐21 cells (Nishimoto et al. '78). At the non‐permissive temperature of 39.5°C, inhibition of ^3^H‐thymidine into acid‐precipitable material begins 1 to 2 h after the cells are released from a block at the
## Abstract Viability, DNA synthesis and mitosis have been followed in the temperature sensitive Chinese hamster cell mutant K12 under permissive and non‐permissive conditions. On incubation at 40°C cells retained their ability to form colonies at 33°C for 15 to 20 hours, but viability was lost gra