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Studies on long chaincis- andTrans-acyl-CoA esters and acyl-CoA dehydrogenase from rat heart mitochondria

✍ Scribed by G. O. Korsrud; H. B. S. Conacher; G. A. Jarvis; J. L. Beare-Rogers


Book ID
112771767
Publisher
Springer-Verlag
Year
1977
Tongue
English
Weight
331 KB
Volume
12
Category
Article
ISSN
0024-4201

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A fast and versatile method for extracti
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A method for the extraction of acyl-CoA esters from tissue, and their subsequent analysis by HPLC is described. The lipids are removed by a two-phase extraction in a chloroform/methanol/water system. The long-chain acyl-CoA esters are extracted using methanol and a high salt concentration (2 M ammon