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Studies on extracellular and intracellular purified amylases from a thermophilic Bacillus stearothermophilus

โœ Scribed by R.A.K. Srivastava


Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
427 KB
Volume
6
Category
Article
ISSN
0141-0229

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Cloning and expression of a thermophilic
โœ Tsukagoshi, N. ;Ihara, H. ;Yamagata, H. ;Udaka, S. ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Springer ๐ŸŒ English โš– 584 KB

A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII