Purification and partial characterizatio
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M. P. Thompson; J. Larner; Daniel L. Kilpatrick
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Article
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1984
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Springer
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English
โ 607 KB
An insulin mediator which inhibits cAMP-dependent protein kinase has been purified approximately 1000-2000-fold from skeletal muscle. Following heat treatment, charcoal adsorption and Sephadex G-25 sieving, Sephadex G-15 sieving and HPLC over an anion exchange column were performed. The mediator has