Studies on chitosan: 1. Determination of the degree of N-acetylation of chitosan by ultraviolet spectrophotometry and gel permeation chromatography
β Scribed by Sei-ichi Aiba
- Book ID
- 118146804
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 348 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0141-8130
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## Abstract Firstβderivative UV spectrophotometry is shown to be a reliable method for the determination of the degree of __Nβ__acetylation of chitosan samples. A mathematical expression is derived that allows to determine the DA directly from the mass concentration of a chitosan solution and the f
A simple and rapid method for the quantitative determination of N-acetyl groups in chitin-chitosan samples consists of an acid hydrolysis conducted at high temperature with a mixture of sulfuric and oxalic acids. The acetic acid formed is determined by spectrophotometry at 210 nm after separation fr
The composition and sequence of 2-acetamido-2-deoxy-beta-D-glucose (GlcNAc) and 2-amino-2-deoxy-beta-D-glucose (GlcN) residues in partially N-deacetylated chitosans, prepared under homogeneous and heterogeneous conditions, have been determined by 1H-n.m.r. spectroscopy. It was necessary to depolymer