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Studies on Brucella interferon: Chromatographic behaviour and purification

✍ Scribed by Catherine Bousquet-Ucla; Juana Wietzerbin; E. Falcoff

Book ID
105154127
Publisher
Springer Vienna
Year
1980
Tongue
English
Weight
953 KB
Volume
63
Category
Article
ISSN
1432-8798

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The separation of ~'-5' dinuclcoside monophosphates from their corresponding 3'-5' isomers on QAE-Sephadex A-25 (bicarbonate form) is described. The column is loaded with a mixture of the two isomers and eluted with a linear gradient of ammonium bicarbonate (from 0.02 to 0.2 M). The two isomers appe