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Further studies on the chromatographic behaviour of dinucleoside monophosphates

✍ Scribed by I. Barzilay; J.L. Sussman; Y. Lapidot

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
572 KB
Volume
79
Category
Article
ISSN
1873-3778

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✦ Synopsis

The separation of ~'-5' dinuclcoside monophosphates from their corresponding 3'-5' isomers on QAE-Sephadex A-25 (bicarbonate form) is described. The column is loaded with a mixture of the two isomers and eluted with a linear gradient of ammonium bicarbonate (from 0.02 to 0.2 M). The two isomers appear in two different peaks, and the nucleotidic material is isolated by lyophilization.

In addition, the separation of 2'3' dinucleoside monophosphates from the corresponding 3'-s'isomers by paper chromatography and by thin-layer chromatography on aluminium cards coated with cellulose powder is described.

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