Studies on attachment and spreading of human keratinocytes in serum-free medium
β Scribed by TENCHINI, M; RANZATI, C; MALCOVATI, M
- Book ID
- 122332635
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 128 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0309-1651
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π SIMILAR VOLUMES
Growth and differentiation of keratinocytes in a serum-free medium (keratinocyte growth medium or KGM) was studied and compared to that under conditions in which serum and feeder cell layers were used. Cells were grown in KGM containing 0.1 m M calcium (KGM/low calcium), KGM containing 1.2 m M calci
Keratinocytes and fibroblasts isolated from human neonatal foreskin can be plated and grown through multiple rounds of division in vitro under defined serum-free conditions. We utilized these growth conditions to examine the mitogenic potential of acidic and basic fibroblast growth factor (aFGF and