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Reactivity of monoclonal anti-human skin basal cell antibody to cultured keratinocytes in serum-free medium

โœ Scribed by FURUKAWA, F; NORRIS, D; KASHIHARASAWAMI, M; LYONS, M; UEDA, M; IMAMURA, S


Book ID
122829270
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
758 KB
Volume
1
Category
Article
ISSN
0923-1811

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Potassium or sodium phosphate was found to stimulate the production of human monoclonal antibody by human-human hybridoma HB4C5. The addition of 15 mM Na-phosphate (pH 7.4) into serum-free culture medium increased the antibody production up to 4-fold, when seeded at cell density of 1 x 10 5 cells/ml