The non-cellulosic polysaccharides of flax fibre cells were isolated using chemical extraction methods. Extraction of mature retted flax fibre with the calcium chelating agent trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (CDTA) removed polysaccharides enriched in galacturonic acid. These
Studies of the polysaccharides of Populus alba L.: Isolation and characterisation of xyloglucans
✍ Scribed by Štefan Karácsonyi; Vladimír Kováčik
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 749 KB
- Volume
- 185
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
Extraction with alkali of the polysaccharides from the tissue culture of primary cell walls of poplar (Populus dba L.) gave the major fraction of hemicellulose B, from which two homogeneous xyloglucans were isolated by fractionation using ion-exchange and gel-permeation chromatography followed by precipitation with Fehling's solution. These xyloglucans had highly branched structures which differed in the degree of side-chain substitution, but the basic structure was similar to those of xyloglucans isolated from other dicotyledons.
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A method for isolation of flax RNA is described; properties of the isolated RNA are given. RNA degradation was held to a minimum through a high pH (9.5) extraction buffer, diethylpyrocarbonate (4%) as a nuclease inhibitor, a high concentration (1.5%) of sodium dodecylsulphate, 2 mM Mg", and separati