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Studies of the mechanism of spleen cell cure for osteopetrosis inia rats: Appearance of osteoclasts with ruffled borders

✍ Scribed by Marks, Sandy C.

Book ID
102697575
Publisher
Wiley (John Wiley & Sons)
Year
1978
Tongue
English
Weight
913 KB
Volume
151
Category
Article
ISSN
0002-9106

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## Abstract A neutral protease was extracted from rat ascites hepatoma cells and effectively purified by fractionation with ammonium sulfate followed by chromatography using TEAE‐cellulose and hydroxylapatite in that order. The protease eluted in 120 mM phosphate buffer (pH 6.8) on hydroxylapatite,