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Structures and amounts of branched molecules in rice amyloses

โœ Scribed by Yasuhito Takeda; Susumu Hizukuri; Bienvenido O. Juliano


Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
243 KB
Volume
186
Category
Article
ISSN
0008-6215

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โœฆ Synopsis


Rice starches with low (japonica varieties) and high (indica varieties) affinities for iodine have similar contents of amylose but different affinities of their amylopectins for iodine'. Their amyloses have the same affinity for iodine and show no significant differences in molecular size, average chain-length, and number of chains2. However, the structures and amounts of their branched molecules have not been examined except for the amylose from a japonica, Sasanishiki variety3. We now report on five rice amyloses from starches with low [japonica: Hokkaido (unknown variety, produced in Hokkaido), and Sasanishiki] and high (indica: IR32, IR36, and IR42) affinities for iodine.

No method is available at present for the isolation of branched amyloses. Therefore, the beta-limit dextrins (P-LD) were isolated3 after treatment of the amylose fraction with beta-amylase in order to remove all the linear molecules, and their properties are recorded in Table I. The iodine staining and binding properties


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Pure amyloses obtained from various starches contain limited numbers of branch linkages and are mixtures of branched and unbranched moleculesl-14, although amyloses are defined as unbranched molecules. However, amyloses of various origins have been characterised generally without separation of the b