The structures of branched and linear molecules of the isolated amylose (number-average dp 850) from rice were examined by the tritium labeling method and gel-permeation chromatography. The branched and linear molecules showed similar, symmetrical distributions on a molar basis with a dp of 710 and
Structures and amounts of branched molecules in rice amyloses
โ Scribed by Yasuhito Takeda; Susumu Hizukuri; Bienvenido O. Juliano
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 243 KB
- Volume
- 186
- Category
- Article
- ISSN
- 0008-6215
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โฆ Synopsis
Rice starches with low (japonica varieties) and high (indica varieties) affinities for iodine have similar contents of amylose but different affinities of their amylopectins for iodine'. Their amyloses have the same affinity for iodine and show no significant differences in molecular size, average chain-length, and number of chains2. However, the structures and amounts of their branched molecules have not been examined except for the amylose from a japonica, Sasanishiki variety3. We now report on five rice amyloses from starches with low [japonica: Hokkaido (unknown variety, produced in Hokkaido), and Sasanishiki] and high (indica: IR32, IR36, and IR42) affinities for iodine.
No method is available at present for the isolation of branched amyloses. Therefore, the beta-limit dextrins (P-LD) were isolated3 after treatment of the amylose fraction with beta-amylase in order to remove all the linear molecules, and their properties are recorded in Table I. The iodine staining and binding properties
๐ SIMILAR VOLUMES
Pure amyloses obtained from various starches contain limited numbers of branch linkages and are mixtures of branched and unbranched moleculesl-14, although amyloses are defined as unbranched molecules. However, amyloses of various origins have been characterised generally without separation of the b