Structure of the polysaccharide S-156 elaborated by Klebsiella pneumoniae ATCC 316 46

✍ Scribed by Anders Johansson; Per-Erik Jansson; Göran Widmalm

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 373 KB
• Volume: 253
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(94)80079-0

✦ Synopsis

Bacterial polysaccharides constitute non-toxic, well-defined, natural polymers which have found many industrial applications. The polysaccharide with the code name S-156 from Klebsiellu pneumoniae ATCC 316 46 has good viscosity properties and is therefore of potential commercial interest. We have now elucidated the structure of S-156, using methylation analysis, computer-assisted analysis, and NMR studies.

Previous qualitative analysis' of S-156 showed galactose, fucose, galacturonic acid, and U-acetyl groups as components. A quantitative analysis using hydrolysis with 0.5 M CF,CO,H gave r&.tcose and p-galactose in the ratio 0.9: 1.0. A small amount of D-glucose was also present, derived from an unknown source as later no signals in the NMR spectra could be assigned to it. A sample that had been treated with methanolic hydrogen chloride also, on GLC analysis, showed o-galacturanic acid. The absolute configurations of the sugars were determined according to Gerwig et al. .

2,3

The 'H NMR spectrum of S-156 showed, inter ah, a signal at