The structure of the capsular polysaccharide elaborated by KlebsieUa type 7 has been investigated. NMR spectroscopy together with sugar and methylation analysis have been the main methods used. A uronic acid degradation was also employed. The polysaccharide consists of hexasaccharide repeating units
Structural studies of the capsular polysaccharide (S-21) from Klebsiella pneumoniae ATCC 31314
✍ Scribed by Per Edebrink; Per-Erik Jansson; Göram Widmalm
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 663 KB
- Volume
- 245
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The structure of the polysaccharide (S-21) elaborated by KZebsielZa pneumoniae ATCC 31314 has been investigated. NMR spectroscopy, sugar and methylation analysis, uranic acid degradation, and partial hydrolysis to oligosacccharides were the main methods used. In order to obtain good NMR spectra, the polymer was subjected to non-specific degradation by treatment with fuming hydrochoric acid. It is concluded that S-21 is composed of pentasaccharide repeating units with the following structure.
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