The structure of the O-specific polysaccharide of Klebsiella pneumoniae O1K2 lipopolysaccharide was investigated by use of methylation, periodate oxidation, partial hydrolysis, and 1H- and 13C-n.m.r. spectroscopy. It was shown to consist of a linear chain composed of two disaccharide repeating units
Structure of the O-specific polysaccharide from Bukholderia pickettii strain NCTC 11149
✍ Scribed by Lesley Galbraith; Ruth George; Jürgen Wyklicky; Stephen G. Wilkinson
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 537 KB
- Volume
- 282
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
A polymeric fraction (the putative O antigen) has been isolated from the lipopolysaccharide of the type strain of Burkholderia pickettii. The components of the polymer and their molar proportions were: L-rhamnose (3), 2-acetamido-2-deoxy-D-glucose (1), and O-acetyl (1). By means of NMR studies and chemical degradations, the basic repeating-unit of the polymer was identified as a linear tetrasaccharide of the structure shown. The O-acetyl group is probably located at position 2 of the 3-substituted alpha-L-Rha p. Similar polymers constitute O antigens in the related species Burkholderia solanacearum.
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