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Structure of the De-O-acetylated glucuronoxylomannan from Cryptococcus neoformans serotype D, as determined by 2D NMR spectroscopy

✍ Scribed by Marshall A. Skelton; Robert Cherniak; Leszek Poppe; Herman van Halbeek

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
738 KB
Volume
29
Category
Article
ISSN
0749-1581

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✦ Synopsis

Abstract

The primary structure of the de‐O‐acetylated glucuronoxylomannan isolated from Cryptococcus neoformans serotype D was characterized de novo by NMR spectroscopy. Assignment of the ^1^H and ^13^C spectra of the polysaccharide involved a combination of 2D NMR experiments including double‐quantum‐filtered {^1^H,^1^H} COSY, {^1^H,^1^H} TOCSY and ^1^H‐detected {^1^H,^13^C} heteronuclear multiple quantum coherence spectroscopy. The sequence of the glycosyl residues, including the linkage positions, was deduced by {^1^H,^1^H} rotating‐frame NOE (ROESY) spectroscopy. The serotype‐D glucuronoxylomannan was found to be a branched polymer with a repeating pentasaccharide unit of the following structure: magnified image.

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