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Structure of the carbohydrate portion of the sialoglycopeptide isolated from the skin of the fish Mastacembalus armatus

✍ Scribed by Rina Ghosh; Santosh Kumar Sikder; Amalendu Das

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
805 KB
Volume
162
Category
Article
ISSN
0008-6215

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✦ Synopsis

The sialglycopeptide (PSGP-A), isolated from the degelled skin of the fish Mastacembalus armatus, contained sialic acids (NANA and NGNA, 13.5%), GlcNAc (19.3%), Gal (14.7%), Man (12.6%), Fuc (2.5%), and amino acids of which Asp, Ser, Thr, and Pro preponderated. Affinity chromatography showed that the two sialic acids were integral parts of the same glycopeptide molecule. The carbohydrate portion was linked through GlcNAc to Asn of the peptide core. Methylation analysis of PSGP-A and its degradation products and periodate-oxidation studies indicated a structure for the carbohydrate portion of PSGP-A which resembled those of other sialoglycopeptides having similar carbohydrate compositions.

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