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Structural analysis of the carbohydrate chain of glycopeptides isolated from Robinia pseudoacacia seed lectins

✍ Scribed by Josiane Wantyghem; Nicole Platzer; Mireille Giner; Christian Derappe; Yves Goussault

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 797 KB
Volume: 236
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(92)85015-r

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✦ Synopsis

Robinia pseudoacacia seeds contain lectins which are closely related. Pronase digestion of the dimeric and tetrameric lectins, RPA1 and RPA3, gave glycopeptides. The structure of the oligosaccharide was determined by 1H NMR spectroscopy and carbohydrate determination as alpha-D-Manp-(1-->3)-[beta-D-Xylp-(1-->2)]-[alpha-D-Manp+ ++-(1-->6)]-beta- D-Manp-(1-->4)-beta-D-GlcpNAc-(1-->4)-[alpha-L-Fucp-(1-->3)] -beta-D-GlcpNAc - (1-->4)-Asn. It appears that the 34-kDa constitutive polypeptide of RPA1 contains 4-5 carbohydrate chains whereas the 30.5-kDa and 29-kDa subunits of RPA3 contain two and one oligosaccharide chains, respectively.

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