In addition to a neutral glycan, lipopolysaccharide extracts from the reference strain for Serratia marcescens serogroup O22 contain an acidic polymer which probably defines the serogroup and is of microcapsular origin. The polymer is doubly branched with a heptasaccharide repeating unit and a galac
Structure of an acidic microcapsular glycan from the reference strain (C.D.C. 866-57) for Serratia marcescens serogroup 01
✍ Scribed by Anders Johansson; Göran Widmalm; Per-Erik Jansson; Stephen G. Wilkinson
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 424 KB
- Volume
- 270
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The structure of the acidic polysaccharide from Serratia marcescens serogroup O1 has been investigated. NMR spectroscopy together with sugar and methylation analysis have been used as well as a uronic acid degradation. The polysaccharide consists of pentasaccharide repeating units having the following structure.
2)-Ot
The polysaccharide also contains one equivalent of O-acetyl groups per repeating unit present on, inter alia, a hydroxymethyl group.
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The lipopolysaccharide extract from the cell wall of the reference strain for Serratia marcescens serogroup O18 contained, in addition to a neutral glycan characterised previously, an acidic glycan. Acidity was contributed both by D-glucuronic acid and by 4-O-[(R)-1-carboxyethyl]-D-glucose (4-O-Lac-
A partially acetylated acidic galactorhamnan has been isolated from the lipopolysaccharide of the 02 reference strain (C.D.C. 868-57) of Serratia marcescens. By means of n.m.r. spectroscopy and methylation analysis of the polymer and the products of Smith degradations, the repeating unit of the poly
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